Anti-ERK1/2 (D196) Antibody

This polyclonal rabbit reagent from Antibodies.com recognizes ERK1 and ERK2 (MAPK3 and MAPK1), the ~44 kDa and ~42 kDa MAP kinases that transmit mitogenic and stress signals through the Ras-Raf-MEK-ERK cascade. Reactivity covers human, mouse, and rat samples, supporting comparative signaling studies in standard cell and tissue models.

The unconjugated antibody is supplied as 100 µl at 1 mg/ml in phosphate-buffered saline containing 15 mM sodium azide at approximately pH 7.2. Validated applications include western blot and immunofluorescence, two readouts commonly used to monitor ERK pathway activity, growth-factor responses, and downstream transcriptional activation in cell lysates.

The included sodium azide acts as a preservative and should be removed by dialysis or gel filtration before any live-cell labeling step. Store refrigerated and protect from light to preserve binding activity; the 100 µl vial supports dozens of immunoblots or immunofluorescence experiments at standard working concentrations.