Anti-ERK1 / 2 Antibody

ERK1 (MAPK3, ~44 kDa) and ERK2 (MAPK1, ~42 kDa) are dual-specificity serine/threonine kinases that act as the terminal effectors of the canonical MAPK/ERK signal transduction cascade. They relay signals from activated receptor tyrosine kinases and Ras/Raf/MEK upstream modules to nuclear and cytoplasmic substrates that govern cell proliferation, survival, differentiation, and response to mitogens and cellular stress. This polyclonal reagent from Antibodies.com was raised in rabbit against ERK1 / 2 and recognizes both isoforms, supporting studies of total ERK abundance as well as downstream pathway activation.

The antibody is supplied unconjugated in a 100 µl volume, leaving labeling chemistry and detection system open to the researcher's experimental design. It has been validated for Western blot (WB) and immunohistochemistry (IHC), and shows reactivity with Human, Mouse, and Rat samples, covering the principal mammalian models used in signal-transduction research. This species coverage makes it practical to compare pathway activity across patient-derived material, established cell lines, and rodent model tissues within a single antibody lot.

Typical uses include immunoblotting of cell or tissue lysates to monitor ERK phosphorylation status at the activating Thr202/Tyr204 motif, and IHC staining of fixed sections to localize ERK1 / 2 expression in tissue architecture. Researchers also deploy it as a loading-control companion to phospho-specific ERK antibodies, or as a general marker of MAPK pathway engagement in cancer, neuroscience, and developmental-biology projects. For best results, aliquot the antiserum upon arrival and store refrigerated, avoiding repeated freeze-thaw cycles.