Anti-ERBB2 (phospho Y1248) Antibody

This rabbit polyclonal IgG antibody from Antibodies.com is raised against human ERBB2 (also known as HER2 or neu) and selectively recognizes the receptor only when phosphorylated at tyrosine 1248, a major C-terminal autophosphorylation site that reflects active signaling. Supplied as 100 µl of unconjugated reagent in a single-use vial, the antibody is validated for Western blot detection of endogenous human ERBB2 in cell lysates, resolving full-length receptor at approximately 185 kDa under standard reducing SDS-PAGE conditions.

Because the epitope is a phospho-tyrosine rather than total protein, this reagent distinguishes the activated receptor pool from baseline ERBB2 expression, supporting studies of basal versus ligand-induced or heterodimer-driven HER2 activation, mechanism-of-action work in breast and gastric cancer models, and characterization of resistance to HER2-targeted therapeutics. Affinity purification against the phosphopeptide, with negative selection against the unphosphorylated counterpart, favors specificity for the phosphorylated receptor in Western blot lysates. Intended for research use only; not validated for diagnostic or therapeutic applications.

Recommended handling: store the 100 µl vial frozen at -20 °C in small working aliquots to limit freeze-thaw cycles, keep protected from light during use, and dilute into standard milk- or BSA-blocked buffer for primary incubation on Western blot membranes.