Anti-ERBB2 (phospho Y1248) Antibody

Phosphorylation of the receptor tyrosine kinase ERBB2 (HER2) at tyrosine 1248 is a hallmark of ligand-driven activation and is widely monitored as a readout of receptor signaling output in cancer cell line models. This rabbit polyclonal antibody, supplied by Antibodies.com as a 50 µl vial, is raised against the phospho-Y1248 epitope and is validated for Western blot detection of the activated receptor in human samples. The unconjugated IgG reagent pairs with standard secondary antibodies and chemiluminescent or fluorescent detection chemistries, allowing researchers to track site-specific phosphorylation dynamics following EGF or heregulin stimulation, or in response to small-molecule HER2 inhibitors. Researchers commonly use it to probe BT474, SKBR3, and other HER2-amplified lines, comparing treated versus untreated lysates to confirm pathway engagement. For best results, store the vial at -20 °C in working aliquots to limit freeze-thaw cycles and protect from light. For research use only.