Anti-ErbB2/HER2 (T1242) Antibody

This rabbit polyclonal antibody from Antibodies.com selectively recognizes ErbB2/HER2 when phosphorylated at threonine 1242, a modification associated with receptor tyrosine kinase activation and downstream signaling through MAPK and PI3K cascades. Supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, the 100 µl vial is validated for Western blot (WB) and immunohistochemistry (IHC) workflows. Confirmed reactivity spans human, mouse, and rat samples, supporting use across standard laboratory models and translational research specimens. The polyclonal format increases the probability of binding the phospho-T1242 epitope even when flanking residues vary modestly between species or sample types. For handling, aliquot the antibody upon first use, store refrigerated, and protect from light; avoid repeated freeze-thaw cycles to preserve binding activity over time. Because sodium azide is included as a preservative, the reagent is not appropriate for live-cell or in vivo administration. The unconjugated formulation gives researchers flexibility to pair it with their preferred secondary detection reagent, whether HRP, fluorophore, or biotin conjugates, depending on the chosen application platform.