Anti-ERAP1 Antibody

Researchers investigating antigen presentation, MHC class I biology, and immune regulation can use the Anti-ERAP1 Antibody from Antibodies.com, a rabbit polyclonal IgG reagent raised against Endoplasmic Reticulum Aminopeptidase 1 (ERAP1). This metalloaminopeptidase trims N-terminal peptide extensions in the endoplasmic reticulum and shapes the peptide repertoire loaded onto MHC class I molecules. Supplied as a 200 µl liquid aliquot of unconjugated antibody, the reagent is validated for Western Blot detection of ERAP1 in human and mouse samples.

ERAP1 has attracted sustained research interest because coding variants in the gene are linked to ankylosing spondylitis and to blood pressure regulation, and its expression is altered in several solid tumors. Researchers frequently probe tumor cell lines, primary lymphocytes, or tissue lysates to compare ERAP1 protein levels across genotypes or treatment conditions. The polyclonal format provides multiple epitopes for recognition, which can be useful when evaluating full-length ERAP1 (~110 kDa) versus truncated or processed forms.

For Western Blot, the reagent is applied to denatured, reduced protein separated by SDS-PAGE and transferred to nitrocellulose or PVDF, where signal is developed with chemiluminescence or colorimetric HRP-conjugated secondary antibodies. Refrigerate the vial on arrival and avoid repeated freeze-thaw cycles to preserve binding activity. The unconjugated format allows users to pair it with their preferred secondary antibody and detection system, and the IgG isotype is compatible with standard anti-rabbit secondary reagents.