Anti-ERAB Antibody

ERAB (also known as HSD17B10 or ABAD) is a 27 kDa mitochondrial enzyme involved in fatty acid β-oxidation, steroid metabolism, and the pathological interaction with amyloid-β implicated in Alzheimer's disease research. The Anti-ERAB Antibody from Antibodies.com is a rabbit polyclonal reagent that specifically recognizes this target and is validated for western blot (WB) and immunohistochemistry (IHC) workflows. Supplied as 100 µl of unconjugated antiserum, the antibody shows reactivity against human, mouse, and rat samples.

The polyclonal format allows recognition of multiple epitopes on the target protein, which can be advantageous when working with samples where post-translational modifications may influence single-epitope binding. As an unconjugated reagent, the antibody can be paired with HRP, fluorophore, or biotin secondary conjugates selected to match the experimental readout. Storage in working aliquots at refrigerator temperature preserves activity for routine use, with longer-term storage at -20°C recommended to limit freeze-thaw degradation.

For experimental design, standard western blot loading amounts of 20-30 µg of total protein from cultured cells or tissue lysates are sufficient for detection, while immunohistochemistry on formalin-fixed paraffin-embedded sections often benefits from antigen retrieval in citrate buffer (pH 6.0) for optimal signal. The human-mouse-rat reactivity profile makes this reagent applicable across translational studies bridging rodent models of neurodegeneration and human clinical specimens.