Anti-ERα Antibody

This rabbit polyclonal antibody from Antibodies.com targets human Estrogen Receptor alpha (ERα), a 67 kDa nuclear hormone receptor that mediates estrogen signaling and is a key marker in hormone-receptor-positive breast cancer research. The unconjugated reagent is supplied as a 100 µl liquid aliquot validated for Western blot, immunohistochemistry, and ELISA workflows, giving laboratories flexibility across protein, tissue, and quantitative detection assays.

Researchers use this reagent to probe ERα expression in human cell lysates and formalin-fixed tissue sections, with common positive-control lines including MCF-7 and T47D breast carcinoma models. The polyclonal format broadens epitope coverage across the receptor, supporting robust detection of the full-length 66 kDa protein in denatured Western blot samples and nuclear staining patterns in IHC.

Compatible with standard HRP- or fluorophore-conjugated secondary antibodies, the antibody integrates into chromogenic, immunofluorescence, and ELISA readouts. For storage, keep refrigerated and protect from light to preserve activity through the shelf life of the preparation, which is intended for research use only.