Anti-ERα (phospho-Y537) Antibody

Raised in rabbit against the phosphorylated tyrosine 537 residue of Estrogen Receptor alpha, this polyclonal reagent from Antibodies.com is targeted to an activation mark located within the hormone-binding domain of ERα. Phosphorylation at Y537 stabilizes the agonist conformation and drives ligand-independent transcriptional activity, making this antibody useful for studies of tamoxifen-resistant breast cancer, MCF7 signaling models, and hormone-driven tumor progression in xenograft and patient-derived tissue samples.

The unconjugated antibody is supplied as 100 µl at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, and is validated for Western blot and immunohistochemistry on human, mouse, and rat samples. Storage at -20 °C in working aliquots is recommended, with limited freeze-thaw cycles to preserve phospho-specific binding. The polyclonal format provides broader epitope coverage around the phospho-Y537 site, supporting detection of the modified receptor under varying denaturation and fixation conditions typical of IHC and immunoblotting workflows.