Anti-ERα (H112) Antibody

Raised in rabbit against the N-terminal region of human Estrogen Receptor alpha, this polyclonal reagent from Antibodies.com supports studies in hormone-responsive cancer biology, reproductive tissue analysis, and nuclear receptor signaling. The H112 epitope maps upstream of the DNA-binding domain, a region frequently targeted in breast cancer research where ERα status guides clinical interpretation.

Supplied as 100 µL of antibody at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide, approximately pH 7.2. The unconjugated format allows flexible pairing with secondary detection reagents in western blot and immunohistochemistry workflows. Verified reactivity spans human, mouse, and rat samples, enabling comparative studies across model systems and archived tissue cohorts.

Aliquot upon receipt and freeze at -20°C for longer term storage to preserve activity. Avoid repeated freeze-thaw cycles. Each vial is intended for research use only and is suited to chromogenic and fluorescent IHC protocols on formalin-fixed paraffin-embedded sections, as well as standard immunoblotting of cell and tissue lysates.