Anti-ERα Antibody

Raised in rabbit against the estrogen receptor alpha (ERα) protein, this unconjugated polyclonal antibody from Antibodies.com is intended for western blot detection of the receptor in human, mouse, and rat samples. The reagent recognizes the nuclear hormone receptor encoded by ESR1, which mediates the transcriptional response to estrogen and serves as a key marker in breast cancer pathology and hormone signaling research. It is supplied as a 100 µl liquid formulation at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a cryoprotectant, and should be stored frozen in aliquots to limit freeze-thaw cycles of the working stock. Researchers commonly use this reagent to probe ERα expression across mammary carcinoma cell lines, reproductive tissue lysates, and model organism samples where the receptor's role in proliferation, differentiation, and endocrine response is under investigation. For western blot, the antibody is typically applied to nitrocellulose or PVDF membranes after SDS-PAGE separation of whole-cell or nuclear extracts, allowing comparison of receptor levels across treated versus untreated conditions or between wild-type and knockout backgrounds.