Anti-Epsin 2 Antibody

A rabbit polyclonal reagent from Antibodies.com recognizes the Epsin 2 protein (gene EPN2), a clathrin-coated pit accessory factor that promotes membrane curvature and the scission of clathrin-coated vesicles during endocytosis. The unconjugated antibody is supplied at 100 µl per vial and is validated for Western blot, immunohistochemistry, and ELISA workflows on human, mouse, and rat samples.

Epsin 2 carries an ENTH (Epsin N-terminal Homology) domain that binds phosphatidylinositol 4,5-bisphosphate at the inner leaflet of the plasma membrane, helping deform the lipid bilayer and recruit clathrin adaptors. Researchers studying synaptic vesicle recycling, Notch receptor trafficking, growth factor receptor internalization, and clathrin-mediated endocytosis in cancer cell lines use the antibody to follow endogenous Epsin 2 levels in whole-cell lysates and fixed tissue sections. Polyclonal recognition of multiple epitopes is particularly useful in immunohistochemistry, where aldehyde fixation can mask individual linear epitopes, and in Western blot of post-translationally modified receptors.

For handling, store the antibody frozen at -20 °C in working aliquots to limit freeze-thaw cycles, and protect it from light. The 100 µl pack size accommodates multiple Western blot or IHC experiments, and the unconjugated format lets the user pair it with a secondary reagent of their choice, including HRP, Alexa Fluor, or biotin conjugates. This reagent is for research use only and is not intended for diagnostic or therapeutic applications.