Anti-EPHX2 Antibody

EPHX2 encodes soluble epoxide hydrolase (sEH), a bifunctional enzyme that plays a central role in epoxy fatty acid metabolism by hydrolyzing anti-inflammatory epoxyeicosatrienoic acids (EETs) into their less active diol forms. This unconjugated rabbit polyclonal antibody from Antibodies.com is validated for Western blot and immunohistochemistry workflows across human, mouse, and rat samples, supporting studies in cardiovascular biology, xenobiotic metabolism, and inflammatory signaling pathways.

The reagent is supplied as 100 µl of affinity-purified antibody at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a stabilizing cryoprotectant. The polyclonal format recognizes multiple epitopes across the EPHX2 target, which is advantageous when probing native protein conformations or detecting lower-abundance isoforms in tissue lysates. Researchers investigating sEH inhibitors for hypertension, cardiac injury, neuropathic pain, or metabolic dysfunction use this reagent to track enzyme expression and subcellular localization.

For storage, the recommended condition is to aliquot and freeze at -20°C to minimize freeze-thaw cycles and preserve antibody activity, with the sodium azide acting as a microbial preservative for short-term bench use. The unconjugated format allows end users to conjugate the antibody to HRP, biotin, or fluorophores of their choice, enabling compatibility with standard chromogenic DAB IHC protocols as well as fluorescence-based detection platforms.