Anti-Ephrin-B2 (Ab-330) Antibody

Manufactured by Antibodies.com, this rabbit polyclonal reagent is raised against a synthetic peptide spanning amino acids 328–332 (N-I-Y-Y-K) of human Ephrin-B2 (EFNB2), allowing it to detect endogenous levels of total Ephrin-B2 protein across human, mouse, and rat samples. Supplied at 1.0 mg/mL in 50 µL aliquots in phosphate-buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol, the antibody is unconjugated and is affinity-purified by epitope-specific peptide chromatography. It is validated for ELISA, Western blotting (recommended dilution 1:500–1:000), immunohistochemistry, and immunocytochemistry/immunofluorescence (recommended dilution 1:100–1:200), making it well suited to a broad range of cell- and tissue-based assays where the predicted 37 kDa Ephrin-B2 band or staining pattern must be resolved cleanly. The product is intended for research use only and is shipped at 4 °C; upon arrival it should be aliquoted and stored at –20 °C, with repeated freeze/thaw cycles avoided to preserve activity. Lead time is typically 5–8 business days, and bulk or custom sizes are available on request.