Anti-EphB3 (phospho-Y608) Antibody

This rabbit polyclonal antibody from Antibodies.com specifically recognizes EphB3 when phosphorylated at tyrosine 608 (phospho-Y608), an autophosphorylation site within the activation loop of the EphB3 kinase domain. EphB3 is a member of the Eph receptor tyrosine kinase family, and signaling through this receptor governs cell sorting, axon guidance, intestinal epithelial boundary formation, and contact-mediated inhibition of proliferation, with documented roles in colorectal carcinoma progression, glioma invasion, and angiogenic remodeling.

The reagent is supplied as 100 µl of affinity-purified antibody at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol, providing a stable stock that tolerates routine freeze-thaw cycles. The unconjugated format allows the end user to pair the antibody with their preferred HRP, fluorescent, or biotinylated secondary reagents for Western blot detection of activated EphB3 in human, mouse, and rat samples.

Researchers commonly use this reagent to compare basal versus ligand-stimulated receptor activation, for example by treating cultured cells with clustered ephrin-B ligands prior to lysis and probing total cell lysates. Storage at -20 °C in small working aliquots is recommended to preserve phospho-specific reactivity across multiple experiments.