Anti-EPHB3 Antibody

Raised in rabbit and affinity-purified to greater than 95% purity by SDS-PAGE, this unconjugated polyclonal IgG reagent from Antibodies.com targets the 998-amino acid receptor tyrosine kinase EPHB3 (Ephrin type-B receptor 3), also known by the aliases HEK2, ETK2, and TYRO6 (UniProt P54753). The antibody is supplied as a 50 µl liquid aliquot, validated for Western blot detection of endogenous EPHB3 in human, mouse, and rat samples, where the target migrates at an observed molecular weight of approximately 110 kDa against the predicted 130 kDa full-length protein. Researchers studying colorectal cancer biology, intestinal stem cell niche maintenance, axon guidance, and vascular remodeling commonly probe EPHB3 with this reagent in epithelial, neuronal, and endothelial lysates; published work has tied EPHB3 silencing to the adenoma-carcinoma transition and to SNAIL1-driven epithelial-mesenchymal transition in CRC cell models. For best results, store the vial refrigerated at 4°C, aliquot upon first use to limit repeated freeze-thaw cycles, and pair with an HRP- or fluorophore-conjugated anti-rabbit IgG secondary reagent in standard immunoblotting workflows. This reagent is intended for research use only and is not approved for diagnostic or therapeutic applications.