Anti-EphA5 (Y515) Antibody

EphA5 belongs to the Eph family of receptor tyrosine kinases, the largest receptor tyrosine kinase subfamily, and contributes to nervous system patterning, axon guidance, and cell migration through ephrin-mediated contact signaling. The polyclonal reagent from Antibodies.com specifically recognizes EphA5 when phosphorylated at the Y515 residue, a regulatory tyrosine in the intracellular region whose modification modulates downstream kinase activity and contact-dependent repulsion responses in neurons and other EphA5-expressing cell types.

Provided as a 100 µl liquid aliquot at 1 mg/ml in phosphate buffered saline (pH 7.2) with 0.05% sodium azide included as a preservative, this unconjugated antibody is raised in rabbit and validated for Western blot detection of endogenous EphA5 protein. Reactivity has been confirmed against human, mouse, and rat samples, enabling cross-species comparisons in neuroscience, developmental biology, and cancer research where altered EphA5 expression has been documented. Intended for research use only, the PBS-based formulation supports direct dilution into standard Western blot workflows on whole-cell lysates prepared under conventional lysis conditions.