Anti-EPCAM Antibody

Antibodies.com offers a rabbit polyclonal antibody raised against EPCAM (EpCAM), supplied as a liquid reagent. The antibody is unconjugated, of the IgG isotype, and is intended for use in Western blot (WB) and immunohistochemistry (IHC), with the manufacturer's product data additionally listing immunocytochemistry/immunofluorescence (ICC/IF) among its validated applications. Reactivity has been demonstrated against human, mouse, and rat samples.

The immunogen is a recombinant fusion protein containing a sequence corresponding to amino acids 24–265 of human EpCAM (NP_002345.2), and the expected molecular weight of the target is 35–45 kDa. The product is supplied in Phosphate Buffered Saline (pH 7.3) containing 50% glycerol and 0.05% Proclin 300, and has been purified by affinity chromatography. Concentration is lot-specific. Recommended working dilutions are 1:500–1:1,000 for WB and 1:50–1:200 for ICC/IF.

The antibody is shipped at 4 °C. Upon delivery it should be aliquoted and stored at –20 °C, with repeated freeze/thaw cycles avoided. It is classified as a primary antibody within the supplier's catalogue.