Anti-EPAS-1 Antibody

Supplied by Antibodies.com, this rabbit polyclonal reagent targets endothelial PAS domain-containing protein 1 (EPAS-1), also known as HIF-2-alpha, and is offered as a 50 µL aliquot of liquid formulated in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide at a working concentration of 1 mg/mL. The antibody is unconjugated and of the IgG isotype, and it was affinity-purified from rabbit antiserum by affinity-chromatography against an epitope-specific immunogen corresponding to a synthesized peptide derived from human EPAS-1 around the non-acetylation site of K385, allowing it to detect endogenous levels of the target. It is validated for Western blot (WB) and ELISA applications, and its reactivity profile has been confirmed in Human, Mouse and Rat samples, making it a versatile option for cross-species studies of hypoxia-inducible signaling. For handling, the vial is shipped at 4 °C and should be aliquoted and stored at –20 °C upon arrival, with repeated freeze/thaw cycles avoided to preserve performance. As a research-use-only reagent intended for laboratory investigation rather than diagnostic or therapeutic use, this polyclonal antibody provides a reliable tool for researchers probing EPAS-1/HIF-2-alpha expression and regulation in cell and tissue models.