Anti-eNOS (phospho T495) Antibody

Raised in rabbit against the phosphorylated Thr495 residue of endothelial nitric oxide synthase (eNOS, NOS3), this polyclonal IgG reagent from Antibodies.com is supplied as a 50 µl aliquot of unconjugated serum. It is validated for Western blot detection of eNOS when phosphorylated at the inhibitory T495 site, a modification linked to protein kinase C signaling and altered vascular reactivity. The antibody recognizes the human and mouse orthologs, making it useful in endothelial cell lysates, aortic tissue homogenates, and cardiovascular disease models where dysregulated NO production is under investigation.

Recommended storage is at -20 °C in working aliquots to limit freeze-thaw cycles, and the reagent is intended for research use only. The phospho-specific recognition is achieved by immunization with a phosphopeptide corresponding to residues surrounding Thr495 of human eNOS, allowing selective detection of the post-translationally modified form versus the total enzyme pool. End users commonly pair this reagent with an anti-total eNOS loading control or with lambda phosphatase-treated lysates to confirm that the observed signal is genuinely phosphorylation-dependent rather than a baseline cross-reactivity artefact.