Anti-eNOS (phospho T495) Antibody

This rabbit polyclonal IgG antibody from Antibodies.com targets eNOS phosphorylated at threonine 495, an inhibitory modification site on endothelial nitric oxide synthase. The unconjugated reagent is validated for western blot detection of phospho-eNOS in human and mouse samples, supplied as a 200 µl vial for research use. Phosphorylation at T495 is recognized as a key regulatory switch that dampens eNOS catalytic activity, so immunological detection of this specific post-translational mark is widely used to probe endothelial signaling, vascular function, and nitric oxide pathway dynamics. The antibody supports western blot workflows in cardiovascular studies, models of endothelial dysfunction, and investigations of altered nitric oxide production in disease, where discriminating the inhibited phospho-state from total eNOS is essential for interpreting pathway activity.