Anti-Enolase Antibody

Manufactured and supplied through the Antibodies.com catalogue under the AFMS distribution channel, this polyclonal reagent is raised in rabbit against Enolase and is supplied at a working concentration of 1 mg/ml in a 50 µl liquid volume. The immunogen is a synthesized peptide derived from the C-terminal region of human Enolase, and the antibody has been affinity-purified from rabbit antiserum by affinity-chromatography using the epitope-specific immunogen. As an unconjugated preparation, it is formulated in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, and is shipped ready to dilute directly into standard assay buffers. Recommended storage is at −20 °C, where it remains stable for up to one year from the date of dispatch. The host species is rabbit, the clonality is polyclonal, and the conjugate state is unconjugated, making the reagent suitable for secondary-labelling workflows and for detection across a range of imaging and immunoassay platforms. Validated applications listed on the Antibodies.com product page include Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF) and ELISA, and reactivity has been confirmed in human, mouse and rat samples, providing broad cross-species coverage for translational and basic-research projects alike. Antibody specificity is described as detecting endogenous levels of Enolase protein, supporting its use in studies of glycolytic enzyme expression, stress responses and tissue-distribution analyses. Typical Western Blot validation data included by Antibodies.com illustrate detection in HepG2 cells and in a panel of additional cell lines, providing a useful reference for expected band patterns. The product is intended for research use only and is not validated for diagnostic or therapeutic applications. Researchers seeking a flexible, unconjugated polyclonal anti-Enolase reagent with multi-application and multi-species utility will find this antibody a convenient option within the Antibodies.com primary antibody range, available at the 50 µl pack size to support pilot and mid-scale experimental programmes.