Anti-Enolase Antibody

Raised in rabbit as a polyclonal antiserum and supplied unconjugated, this laboratory reagent targets enolase, a conserved dimeric glycolytic metalloenzyme widely used as a read-out of cellular metabolic activity in basic research. The 100 µl volume is sized for multi-assay projects, and the antibody is validated for western blot, immunohistochemistry, immunofluorescence, and ELISA workflows, so the same primary can be carried from membrane-based screening to fixed-tissue localization and quantitative plate-based detection. The polyclonal format broadens epitope coverage across the target, which is helpful when probing denatured proteins on blots or formalin-fixed paraffin-embedded specimens where a single monoclonal epitope may be masked. Reactivity has been demonstrated with human, mouse, and rat samples, supporting translational studies that pair rodent model data with clinical tissue. As an unconjugated primary, it pairs readily with secondary antibodies labeled to HRP, biotin, Alexa Fluor dyes, or gold particles matched to the chosen detection platform. Aliquoting on receipt helps limit freeze-thaw cycles and preserves lot-to-lot consistency across long-running studies. Antibodies.com ships this reagent for research use only, intended for laboratory investigation of enolase expression, localization, and function in cells and tissues.