Anti-ENO1 Antibody

Alpha-enolase (ENO1) is the metalloenzyme responsible for converting 2-phosphoglycerate to phosphoenolpyruvate during glycolysis, and this rabbit polyclonal antibody from Antibodies.com is raised against the ENO1 target for detection in Western blot, immunohistochemistry, and immunofluorescence workflows. Supplied as 100 µL of unconjugated antiserum, the reagent cross-reacts with Human, Mouse, and Rat samples, supporting translational studies that span cell-line models, primary tissue, and rodent disease systems.

Beyond its canonical cytoplasmic role in energy metabolism, ENO1 is a multifunctional protein that relocalizes to the cell surface in many cell types, where it acts as a plasminogen receptor. Altered ENO1 expression or surface relocalization has been linked to tumor progression, the cellular hypoxia response, and the generation of anti-ENO1 autoantibodies associated with autoimmune vasculitis. Researchers therefore use this reagent to probe ENO1 abundance and subcellular localization in cancer biology, neuroscience, and immunology investigations.

Aliquot upon receipt to limit freeze-thaw cycles and store at -20 °C for long-term stability. The unconjugated format allows pairing with horseradish peroxidase-conjugated, biotinylated, or fluorophore-conjugated secondary antibodies matched to the rabbit host, giving flexibility across chromogenic immunohistochemistry, immunofluorescence imaging, and chemiluminescent Western blot detection of the approximately 47 kDa ENO1 band.