Anti-Endoplasmin Antibody

Endoplasmin, also referred to as GRP94 and encoded by the HSP90B1 gene, is a stress-responsive endoplasmic reticulum chaperone of the HSP90 family. This unconjugated rabbit polyclonal reagent is raised against the endogenous human and mouse protein and is supplied by Antibodies.com as a 100 µl aliquot. It supports multiple immunodetection workflows including Western blot, immunohistochemistry, immunofluorescence, and ELISA, and is provided as a primary antibody that recognizes the native target without an attached reporter.

The reagent tolerates standard immunolabeling protocols and is suited to studies of ER homeostasis, unfolded protein response signaling, chaperone-mediated peptide loading onto MHC class I molecules, and the role of GRP94 in tumor immunogenicity and viral glycoprotein maturation. Because it is polyclonal, the antibody recognizes multiple epitopes on the target, which can be helpful when probing denatured lysates on Western blot as well as fixed tissue sections in IHC.

Endoplasmin resides in the ER lumen, so successful detection usually requires permeabilization of fixed cells for immunofluorescence, or preparation of membrane-containing fractions for Western blot. Researchers working on secretory pathway function, ER stress biology, or cell surface GRP94 will find this reagent useful for tracking expression changes following pharmacological perturbation of cells. The unconjugated format enables flexible pairing with user-selected secondary antibodies and detection chemistries, including HRP, biotin, Alexa Fluor, and DyLight conjugates, across blotting, imaging, and chromogenic readouts.