Anti-ELSPBP1 Antibody

This rabbit polyclonal antibody from Antibodies.com targets ELSPBP1 (Epididymal Sperm-Binding Protein 1), a secreted glycoprotein predominantly expressed in the human epididymis where it contributes to sperm maturation and post-testicular maturation events. The unconjugated reagent is supplied as 100 µl of a 1 mg/ml stock in PBS containing 0.1% sodium azide and 50% glycerol, providing a ready-to-use liquid format for Western blot and immunohistochemistry workflows on human samples.

Validated applications cover Western blot detection of the endogenous ELSPBP1 band and immunohistochemistry on formalin-fixed tissue sections. The polyclonal host response provides recognition of multiple epitopes across the target, an advantage when working with limited tissue or when signal amplification is needed. Sodium azide in the buffer acts as a preservative; the 50% glycerol component prevents freeze-thaw damage, so the vial can be stored at -20°C in working aliquots and thawed on ice for repeated use without loss of activity.

For Western blot, the antibody can be used with HRP-conjugated secondary reagents and chemiluminescent detection. For IHC on paraffin sections, antigen retrieval in citrate buffer pH 6 followed by HRP-DAB chromogenic detection gives clear cytoplasmic staining in epididymal epithelium. Researchers studying male reproductive biology, sperm capacitation, contraceptive target validation, or epididymal-specific gene expression will find this reagent useful for characterizing tissue distribution and confirming localization of the ELSPBP1 gene product at the protein level.