Anti-ELK1 (phospho T417) Antibody - Identical to Abcam (ab194795)

This rabbit polyclonal antibody from Antibodies.com is raised against ELK1 phosphorylated at threonine 417, a regulatory residue within the ETS-domain transcription factor that drives immediate-early gene expression downstream of MAPK/ERK signaling cascades.

Purified as an IgG preparation and supplied unconjugated in a 50 microliter liquid aliquot, the reagent supports direct chromogenic or fluorescent detection in user-optimized workflows. Reactivity spans human, mouse, and rat ELK1, enabling cross-species comparisons across standard mammalian model systems.

Validated applications include immunohistochemistry on formalin-fixed paraffin-embedded tissue sections and immunofluorescence staining for subcellular localization of activated ELK1 in cultured cells. Investigators commonly deploy phospho-T417 ELK1 detection to monitor ERK-pathway activation in growth-factor response studies, neuronal signaling experiments, and tumor biology research where distinguishing active transcription factor pools from total protein is essential.

Standard handling recommends aliquoting upon first use to limit freeze-thaw cycles and preserve phospho-specific binding activity across long-running experiments.