Anti-ELK1 (phospho T417) Antibody - Identical to Abcam (ab194795)

For researchers probing the MAPK/ERK signaling cascade, this rabbit polyclonal IgG antibody selectively recognizes ELK1 only when phosphorylated at threonine 417, a regulatory residue in the C-terminal transactivation domain that is directly targeted by activated ERK1/2. Supplied as 100 µL of unconjugated antiserum, the reagent is validated for immunohistochemistry (IHC) and immunofluorescence (IF) workflows on human, mouse, and rat samples, enabling visualization of pathway-engaged ELK1 in formalin-fixed tissue sections and cultured cells.

Use the antibody to compare basal versus stimulated ERK pathway output, to map spatial distribution of active ELK1 in tumor or neural specimens, or to corroborate phospho-proteomic datasets with morphological readouts. Store refrigerated at 2-8 degrees C in working aliquots to preserve phospho-specific binding and avoid repeated freeze-thaw cycles. Antibodies.com formats each lot for direct use in standard immunostaining protocols with appropriate secondary antibodies, and the polyclonal antibody provides robust signal across the conserved phospho-T417 epitope.