Anti-ELK1 (phospho S389) Antibody

ELK1 is a nuclear transcription factor and core effector of the Ras/MAPK cascade; phosphorylation at serine 389 by ERK1/2 is the activating modification that drives its binding to serum response elements (SRE) and transactivation of immediate-early genes. The Anti-ELK1 (phospho S389) Antibody from Antibodies.com is a rabbit polyclonal reagent raised specifically against this phospho-epitope, allowing researchers to distinguish activated ELK1 from total ELK1 pools in cell signaling studies.

Supplied as 50 µl of unconjugated IgG polyclonal serum, this reagent is validated for Western blot (WB) and immunohistochemistry (IHC) and cross-reacts with Human, Mouse, and Rat samples. The polyclonal format broadens epitope coverage, which is useful when the phosphorylation state is variable across preparations or when signal from low-abundance targets is required. For Western blot, lysates should be prepared with phosphatase inhibitors to preserve the S389 phosphate, then resolved on SDS-PAGE; for IHC, antigen retrieval conditions should be matched to the fixation chemistry of the tissue section being stained.

Aliquot upon receipt and store at -20 °C, protected from light, to limit freeze-thaw degradation of the polyclonal IgG over a typical multi-week project lifespan. This reagent is a focused tool for studies of growth factor signaling, neuronal plasticity, and oncogenic MAPK pathway activation, where the level of S389-phosphorylated ELK1 is the standard readout for active ERK signaling. Pair it with a total ELK1 antibody or upstream MAPK pathway controls to resolve pathway activation state against overall expression level.