Anti-ELK1 (phospho S383) Antibody

Manufactured by Antibodies.com as a research-grade primary antibody, this reagent specifically recognizes ELK1 only when phosphorylated at serine 383, a regulatory site targeted by the ERK/MAPK signaling cascade.

Phosphorylation at S383 drives ELK1 transcriptional activation downstream of growth factor, mitogenic, and stress-related stimuli, so this antibody serves as a widely used readout of ERK pathway activity in cell-based and tissue-based studies.

Produced in rabbits and purified as a polyclonal IgG preparation in unconjugated format, the antibody is supplied as 50 µl aliquots suitable for multiple western blot and immunohistochemistry runs. Cross-validated reactivity against human, mouse, and rat samples enables comparative work on immediate-early gene regulation, neuronal activation, and cancer cell signaling research.

For western blot, lysates should be prepared with phosphatase inhibitors to preserve the phospho-epitope; for IHC, the reagent is applied to fixed tissue sections to localize activated ELK1 at the cellular level. Store frozen at -20 °C in small aliquots to limit freeze-thaw cycles and protect from light. Intended for research use only in in vitro applications.