Anti-EIF5B Antibody

Raised in rabbit against the eukaryotic translation initiation factor 5B (EIF5B) protein, this polyclonal antibody from Antibodies.com is supplied as 100 µl of unconjugated antiserum ready for use in standard laboratory immunodetection workflows. EIF5B is a GTPase that catalyzes ribosomal subunit joining during the final step of translation initiation, making it a useful marker in studies of protein synthesis regulation, ribosome biogenesis, and translational control in mammalian systems.

The antibody was validated for Western blot and immunofluorescence applications, where it detects the endogenous EIF5B band in lysates derived from human, mouse, and rat samples. Researchers commonly apply it to investigate cytoplasmic translation activity, stress response pathways, and the contribution of EIF5B to cell growth and proliferation. The polyclonal format ensures recognition of multiple epitopes across the target, supporting reliable signal in both denaturing (WB) and fixed-cell (IF) conditions. Store aliquots refrigerated between experiments to preserve reagent performance.