Anti-EIF5 Antibody

This primary antibody reagent from Antibodies.com targets eukaryotic translation initiation factor 5 (EIF5), a GTPase that drives hydrolysis of GTP on the 40S pre-initiation complex and promotes joining of the 60S ribosomal subunit during cap-dependent translation in eukaryotic cells.

Raised in rabbit as a polyclonal preparation and supplied at 100 µl per vial in an unconjugated format, the antibody is validated for Western blot (WB) and immunofluorescence (IF) workflows, with confirmed reactivity on human, mouse, and rat samples. The polyclonal composition supports recognition of multiple epitopes on EIF5, which typically yields stronger band intensity in immunoblotting and more consistent cytoplasmic signal in immunofluorescence staining of cultured cells.

For routine use, store refrigerated at 4°C and avoid repeated freeze-thaw cycles; for long-term storage, aliquot the antibody and freeze. The unconjugated preparation allows the reagent to be paired with secondary antibodies of choice, including HRP, Alexa Fluor, or biotin conjugates, depending on the detection platform. This reagent is intended for research use only.