Anti-EIF4G1 (phospho-S1148) Antibody

Targeting the phosphorylated serine 1148 (S1148) site on eukaryotic translation initiation factor 4 gamma 1, this rabbit polyclonal primary antibody from Antibodies.com detects EIF4G1 only in its phosphorylated state. EIF4G1 acts as a central scaffold within the eIF4F cap-binding complex, bridging the cap-bound eIF4E and the eIF4A helicase while recruiting eIF3 to position the 40S ribosomal subunit on capped mRNAs. Modification at S1148 has been linked to mitotic translational control and to signaling events downstream of stress and growth-factor pathways, making phospho-specific detection valuable for studies of cap-dependent translation regulation.

Supplied as 100 µl of antibody at 1 mg/ml in phosphate buffered saline (pH ~7.3) containing 0.05% sodium azide, the product is unconjugated and validated for western blot. It cross-reacts with human, mouse, and rat EIF4G1, supporting work in established cell lines and primary tissue lysates. For stability, store the vial at -20 °C in working aliquots to limit freeze-thaw cycles and protect from light; the azide preservative can be removed or compensated for if downstream applications are azide-sensitive.

Typical workflow involves SDS-PAGE separation of whole-cell or cytoplasmic lysates, transfer to nitrocellulose or PVDF, blocking, overnight incubation with the primary, and detection with an HRP- or fluorophore-conjugated secondary. Pairing this reagent with a total EIF4G1 antibody or a pan-phospho control allows researchers to resolve phosphorylation stoichiometry in mTOR, stress-response, and cell-cycle studies.