Anti-EIF4G1 Antibody

This polyclonal antibody from Antibodies.com targets human EIF4G1, the largest subunit of the eIF4F cap-binding translation initiation complex that scaffolds the small ribosomal subunit onto capped mRNAs through its direct interaction with eIF3. Raised in rabbit, the reagent is supplied as an unconjugated liquid at 1 mg/ml in PBS containing 0.1% Sodium Azide and 50% Glycerol, and is packaged as a 100 µl vial ready for direct use in western blot and immunohistochemistry workflows on human samples.

The reagent recognizes the endogenous scaffolding protein in its full-length form and is suited to studies of translation initiation, IRES-driven translation in picornavirus-infected cells, and EIF4G1 cleavage events triggered by viral 2A and 3C proteases. Laboratories examining cap-dependent versus cap-independent translation, ribosomal recruitment, and oncogenic reprogramming of protein synthesis in human tissues routinely deploy this reagent. Because the host species is rabbit and the target is conserved across human samples, paired detection with mouse or goat secondary antibodies is straightforward in multi-label IHC and immunoblot panels.

Storage: keep the 100 µl vial frozen at -20 °C in working aliquots to limit freeze-thaw cycles of the glycerol-stabilized stock. For short-term bench use, keep refrigerated and protected from light. The unconjugated polyclonal antibody is supplied as a 100 µl vial in liquid format containing PBS, 0.1% Sodium Azide, and 50% Glycerol as cryoprotectant, and is reactive against human EIF4G1 in western blot and immunohistochemistry applications.