Anti-EIF4EBP1 (phospho T37 + T46) Antibody

Phosphorylation at threonine 37 and threonine 46 on eukaryotic translation initiation factor 4E-binding protein 1 (EIF4EBP1) marks the earliest priming events in mTORC1-driven translational control, preceding release of eIF4E and activation of cap-dependent protein synthesis. This unconjugated rabbit polyclonal IgG reagent from Antibodies.com is raised against the doubly phosphorylated EIF4EBP1 epitope and is intended for Western blot use with Human, Mouse, and Rat samples. Each vial contains 50 µl of polyclonal antiserum suitable for probing signaling cascades in cell lysate preparations. The T37 and T46 priming sites gate subsequent phosphorylation at T70 and S65, allowing dissection of upstream mTOR kinase activity versus downstream feedback regulation of cap-dependent translation. The polyclonal IgG backbone tolerates minor sequence variation across species orthologs, and the unconjugated format lets users choose their preferred HRP or near-infrared secondary system for chemiluminescent or fluorescent membrane imaging. Store refrigerated for short-term use, or aliquot and freeze for longer-term storage, avoiding repeated freeze-thaw cycles that erode polyclonal titer over time.