Anti-eIF4E3 Antibody

Eukaryotic translation initiation factor 4E family member 3 (eIF4E3) is one of three cap-binding proteins in the eIF4E family, supporting translation initiation of specific mRNA subsets and remaining comparatively under-characterized relative to eIF4E1. Its expression is notably enriched in testis, where it has been investigated for roles in spermatogenesis, while loss-of-function variants have surfaced in genetic studies of autism spectrum disorder and non-syndromic intellectual disability, broadening the antibody's research relevance into reproductive biology, cancer translation dysregulation, and neurodevelopmental inquiry.

The polyclonal reagent from Antibodies.com is raised in rabbit and supplied as 100 µL of unconjugated antiserum, validated for Western blot and ELISA workflows against human, mouse, and rat samples. The polyclonal format offers broad epitope coverage that aids detection of the endogenous target even when post-translational modification or splice variation shifts a single site, and the unconjugated format preserves flexibility for downstream conjugation to HRP, biotin, or fluorophores.

For best results, aliquot the 100 µL stock upon first thaw and store the working fractions refrigerated, returning them to -20°C for longer holds so that repeated freeze-thaw exposure does not erode titre. Common applications include Western blot analysis of eIF4E3 expression across cell lines and primary tissues, ELISA-based quantification of the endogenous protein, and characterization of eIF4E family members in mechanistic translation studies. The reagent is intended for research use only.