Anti-EIF4E (phospho S209) Antibody

Eukaryotic translation initiation factor 4E (EIF4E) is a central regulator of cap-dependent mRNA translation, and phosphorylation at serine 209 modulates its activity within the mTOR signaling axis. This rabbit polyclonal antibody from Antibodies.com is raised against the phospho-S209 epitope and is supplied as an unconjugated IgG preparation in a 200 µl volume, ready for direct use in western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) workflows on human samples. The polyclonal format recognizes multiple epitopes surrounding the phosphorylated residue, supporting reproducible detection of the activated form of EIF4E in cell lysates and tissue sections. Being unconjugated, the reagent allows flexible pairing with a wide selection of secondary antibodies or custom labels depending on the experimental design, including chemiluminescent, fluorescent, or enzymatic readouts. It is intended for research use only in studies of translational control, oncogenic signaling, and stress-responsive gene expression where distinguishing the active, S209-phosphorylated pool of EIF4E from the total protein is critical. For best results, store at -20 °C in working aliquots to limit repeated freeze-thaw cycles and preserve phospho-specific binding activity over time.