Anti-eIF4E Antibody

Manufactured by Antibodies.com, this affinity-purified Rabbit polyclonal reagent provides specific detection of endogenous eukaryotic translation initiation factor 4E (eIF4E) and is supplied at a working concentration of 1 mg/mL in a 50 µl volume of PBS buffer containing 50% glycerol, 0.5% BSA, and 0.02% sodium azide. The antibody was raised against a synthesized peptide derived from the human eIF4E sequence surrounding the non-phosphorylation site of serine 209, and was subsequently purified from rabbit antiserum by affinity-chromatography using the epitope-specific immunogen, ensuring low background in downstream assays. Validated applications include ELISA, Western blot (WB), and immunohistochemistry (IHC), and the unconjugated format makes the reagent directly compatible with standard HRP- or fluorophore-conjugated secondary detection chemistries. Cross-reactivity has been confirmed against Human, Mouse, Rat, and Monkey samples, supporting its use across a broad range of in vitro and tissue-based research models. For optimal stability, the product should be stored at –20 °C with freeze/thaw cycles avoided, and the polyclonal nature of the reagent ensures recognition of multiple epitopes on the target. Intended strictly for research use only and not for diagnostic or therapeutic applications, this polyclonal primary antibody offers a reliable, well-characterized option for laboratories investigating cap-dependent translation initiation, mTOR signaling, and the broader translational control landscape.