Anti-EIF4B Antibody

The Anti-EIF4B polyclonal reagent from Antibodies.com is raised in rabbit hosts and supports research into translation initiation and mTOR-dependent signaling. EIF4B (Eukaryotic Translation Initiation Factor 4B) acts as an RNA helicase cofactor for eIF4A and serves as a downstream phosphorylation target of mTOR complex 1 and S6 kinases, enabling investigation of cap-dependent translation, oncogenic transformation, and neuronal synaptic plasticity.

Validated across Western blot (WB), Immunohistochemistry (IHC), and Immunofluorescence (IF), this unconjugated antibody recognizes EIF4B in human, mouse, and rat samples. Researchers apply it to map EIF4B expression and phosphorylation status in cancer cell line lysates, primary neuron cultures, and fixed tissue sections, with published use in lymphoma and breast cancer signaling studies.

Supplied as a 100 µl liquid aliquot at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol as a cryoprotectant. Store at -20 °C; aliquot upon receipt to limit freeze-thaw cycles and protect from light. The polyclonal format delivers multi-epitope recognition across denaturing Western blot workflows and fixed-tissue IHC/IF protocols used in cell biology and neuroscience laboratories.