Anti-eIF4A1 Antibody

The target eIF4A1 is a cytoplasmic DEAD-box RNA helicase that functions as a subunit of the eIF4F cap-binding complex, where it unwinds secondary structure in the 5' untranslated region of mRNAs to promote ribosome scanning during translation initiation. Antibodies.com supplies this rabbit polyclonal reagent as an unconjugated, affinity-purified preparation validated for western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, and RNA immunoprecipitation workflows on human, mouse, and rat samples. The antibody is provided at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a 100 ul aliquot, and is recommended for storage at -20 degrees C in single-use aliquots to limit freeze-thaw cycles. With reactivity toward the ~46 kDa helicase, the polyclonal format captures multiple epitopes and supports native-complex pull-downs for RNA-protein interaction studies, ribosome profiling validation, and investigations of cap-dependent translation, stress granule assembly, and translational control in oncogenic signaling contexts.