Anti-EIF3F Antibody

Raised in rabbits against the eukaryotic translation initiation factor 3 subunit F, this unconjugated polyclonal antibody is supplied by Antibodies.com as a 100 µl vial at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol. EIF3F is a core component of the eIF3 complex, a multi-subunit assembly that loads the 40S ribosomal subunit onto mRNA during cap-dependent translation initiation, and the reagent has been validated for Western blot and immunohistochemistry workflows across human, mouse, and rat tissue samples. The 50% glycerol formulation lets the antibody be stored at -20 °C without freeze-thaw damage and keeps it liquid for convenient pipetting at the bench, while the sodium azide acts as a microbial preservative. Researchers typically dilute the stock into an appropriate buffer for immunoblotting of cell lysates or for antigen-retrieval-based IHC staining of fixed sections, where it reveals the endogenous EIF3F band or tissue distribution. Beyond its translation role, EIF3F has been investigated in the context of p27 regulation and emerges as a point of interest in cancer research, making the antibody useful for studies in cell signalling, proliferation control, and ribosome biology.