Anti-EIF3E Antibody

From Antibodies.com, this primary antibody is a rabbit polyclonal reagent raised against EIF3E. It is supplied as an unconjugated IgG antibody and is validated by the manufacturer for Western blotting (WB); the catalogue entry additionally lists immunofluorescence (IF) as an application. The antibody reacts with human and mouse samples.

The immunogen is a recombinant fusion protein corresponding to amino acids 1–445 of human eIF3e (NP_001559.1). The antibody is affinity-purified and supplied as a liquid in phosphate-buffered saline (pH 7.3) containing 50% glycerol and 0.02% sodium azide; concentration is reported as lot specific. The target protein has a reported molecular weight of 52 kDa.

For Western blotting, a recommended working dilution of 1:500-1:2,000 is suggested. The product is shipped at 4 °C; on receipt, it should be aliquoted and stored at -20 °C, avoiding repeated freeze/thaw cycles.