Anti-EIF3C Antibody

This rabbit polyclonal antibody recognizes EIF3C, a subunit of the eIF3 multiprotein complex that drives cap-dependent translation initiation in eukaryotic cells. Supplied by Antibodies.com as a primary antibody reagent, the preparation is provided at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, packaged as a 100 µl vial that supports a long series of immunostaining or blotting experiments.

EIF3C contributes to ribosome loading and mRNA recruitment, and the protein has been investigated in studies of translational control, stress-granule biology, and several proliferative programs where altered protein output is implicated. Validated applications for this reagent are Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF), spanning denatured lysate analysis, fixed-tissue localization, and subcellular imaging of cultured cells. The antibody cross-reacts with human, mouse, and rat samples, supporting cross-species comparative work in common model systems.

For handling, aliquot the vial into single-use portions and store at -20°C to limit freeze-thaw cycles, keeping the stock protected from light during routine use. Working dilutions should be prepared in appropriate blocking buffer immediately before staining. The unconjugated format lets researchers pair the primary with HRP, fluorophore, or biotin-labeled secondary reagents of their choice, matching chromogenic, fluorescent, or amplified detection chemistries to whichever imaging or blotting platform is available in the lab.

Intended for research use only, this polyclonal reagent fits studies of translation initiation factor composition, ribosome loading kinetics, and downstream signaling pathways where eIF3 complex integrity shapes cellular output.