Anti-EIF2AK2 (phospho T451) Antibody

Primary antibodies that distinguish phosphorylated from non-phosphorylated kinase epitopes are essential tools for studying innate antiviral signaling. This unconjugated rabbit polyclonal IgG reagent from Antibodies.com recognizes EIF2AK2 (Protein Kinase R, PKR) only when the threonine 451 activation-loop residue is phosphorylated, and it is supplied in a 50 µl vial format for use on human tissue sections.

The antibody was raised against a synthetic phospho-peptide corresponding to the region spanning amino acids 418–467 of human PKR, an epitope positioned within the catalytic kinase domain. In immunohistochemistry on formalin-fixed, paraffin-embedded specimens, the rabbit polyclonal reagent can be paired with HRP- or fluorophore-conjugated secondary antibodies of the researcher's choosing, since the antibody is supplied without a conjugated reporter. Reagent reactivity is reported for human tissue, with staining patterns consistent with activated PKR localization in stress granules and perinuclear cytoplasmic compartments, supporting studies of viral infection dynamics, interferon-stimulated translational control, and inflammasome engagement.

Aliquot upon first use and store refrigerated; protect from repeated freeze-thaw cycles to preserve phospho-epitope recognition. The polyclonal antibody format is well suited to capturing the modified threonine in archived clinical samples where preservation of phospho-signaling is critical. For research use only.