Anti-EGFR (V1010) Antibody

This rabbit polyclonal antibody from Antibodies.com targets the Epidermal Growth Factor Receptor (EGFR), a 170 kDa transmembrane glycoprotein and founding member of the ErbB family of receptor tyrosine kinases. EGFR sits at the head of signaling cascades that regulate epithelial cell proliferation, survival, and differentiation, and its dysregulation is a focus of cancer research across lung, colorectal, glioblastoma, and head-and-neck tumour models.

Supplied at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide at approximately pH 7.2, the unconjugated reagent is offered as a 50 µl vial suitable for Western blot, immunohistochemistry, and immunofluorescence workflows. The polyclonal format allows the reagent to recognise multiple epitopes on EGFR, which is helpful when probing different isoforms or post-translational modifications of the receptor. Validation has been performed against human and rat samples, supporting use on cell lines, primary cultures, and tissue sections from these species.

Typical laboratory practice for sodium azide-containing antibody liquids is to store refrigerated, aliquot to limit freeze-thaw cycles, and protect from light during working use. Researchers apply this antibody to formalin-fixed paraffin-embedded sections for IHC, to fixed or live cultured cells for IF imaging of receptor localisation and membrane trafficking, and to cell or tissue lysates for Western blot assessment of total EGFR expression.