Anti-EGFR (V1010) Antibody

This unconjugated polyclonal antibody from Antibodies.com targets the Epidermal Growth Factor Receptor (EGFR), a transmembrane receptor tyrosine kinase that regulates cell proliferation, differentiation, and survival signaling cascades. Raised in rabbit, the reagent is supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, and is packaged as 100 µl in a single-use vial.

The antibody has been validated for Western blot, immunohistochemistry, and immunofluorescence workflows and shows reactivity against human and rat tissue samples, supporting translational oncology research and pathway studies in both species.

EGFR (also known as HER1 or ErbB1) is a 170 kDa transmembrane glycoprotein and a member of the ErbB family of receptor tyrosine kinases. Aberrant EGFR signaling through activating mutations or receptor overexpression is implicated in glioblastoma, non-small cell lung cancer, colorectal carcinoma, and head and neck squamous cell carcinoma, making the receptor one of the most heavily investigated targets in molecular oncology. Researchers use anti-EGFR antibodies to map receptor localization, quantify expression levels, and dissect downstream signaling through the RAS-RAF-MEK-ERK and PI3K-AKT-mTOR pathways.

For Western blot, the antibody detects the full-length receptor around 170 kDa. In immunohistochemistry and immunofluorescence on fixed tissue sections, it produces membranous and cytoplasmic staining patterns consistent with the receptor's known subcellular distribution. The polyclonal format provides epitope redundancy, allowing recognition of multiple regions across the receptor's extracellular and intracellular domains and improving detection of minor sequence variations between species.