Anti-EGFR (phospho Y869) Antibody

Raised in rabbit and purified as an IgG polyclonal preparation, this unconjugated reagent from Antibodies.com specifically recognizes the epidermal growth factor receptor (EGFR) when phosphorylated at tyrosine 869, a site whose modification contributes to downstream receptor signaling. Supplied at a volume of 200 µl, the antibody is validated for Western blot (WB) detection of phospho-EGFR in human samples and has not been documented as validated for additional species within the product sheet.

The polyclonal format provides broader epitope coverage around the Y869 phospho-site compared with monoclonal alternatives, supporting detection of transient phosphorylation events in cell lysates prepared from EGF-stimulated cell cultures or in clinical specimens where receptor activation may be sub-stoichiometric. Investigators exploring receptor tyrosine kinase signaling, comparing phosphorylation levels between tumor and matched normal tissue, or profiling downstream engagement of canonical EGFR effector pathways apply phospho-specific antibodies of this kind to distinguish active receptor pools from total EGFR abundance. The IgG isotype permits flexible secondary antibody selection across routine detection chemistries used in Western blot workflows.

For research use only; the reagent is intended for laboratory investigation of EGFR signaling biology and is not validated for diagnostic or therapeutic applications. Storage under refrigerated conditions preserves binding activity across multiple experiments, with aliquoting recommended to limit freeze-thaw cycles over the shelf life of the product.