Anti-EGFR (phospho-Y1148) Antibody

The phospho-EGFR (Y1148) polyclonal antibody from Antibodies.com is a rabbit host reagent configured for immunohistochemistry (IHC) detection of the activated Epidermal Growth Factor Receptor in tissue sections. Tyrosine 1148 is a major C-terminal autophosphorylation site on the EGFR cytoplasmic domain, and probing this residue distinguishes ligand-driven receptor activation from basal receptor expression, a distinction that matters in solid tumor pathology, where phospho-EGFR signal intensity is linked to proliferation, invasion, and response to tyrosine kinase inhibitors.

Supplied as 100 µl of unconjugated antibody at 1 mg/ml in phosphate buffered saline, pH 7.2, with 0.05% sodium azide as preservative, the reagent is validated for reactivity with human, mouse, and rat samples, supporting comparative tissue studies across patient biopsies, xenograft models, and rodent disease systems. Recommended use covers formalin-fixed paraffin-embedded sections as well as frozen tissue, with downstream detection achievable using standard HRP-DAB chromogen or fluorescently labeled secondary antibodies. For routine use, store at 4 °C; for longer term, aliquot and freeze at -20 °C and avoid repeated freeze-thaw cycles to preserve phospho-epitope reactivity. Intended for research use only.