Anti-EGFR (phospho T693) Antibody

Raised in rabbit and affinity-purified against a synthetic phosphopeptide covering amino acids 661-710 of human EGFR around the Thr 693 phospho-acceptor, this unconjugated IgG reagent from Antibodies.com detects the receptor exclusively in its phosphorylated state and is supplied as a 50 µL liquid aliquot in PBS (pH 7.4) with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. It is validated for Western blot detection of phospho-Thr 693 EGFR in human samples and pairs cleanly with standard anti-rabbit HRP or fluorescent secondaries. The Thr 693 site sits within the intracellular juxtamembrane segment whose threonine phosphorylation by protein kinase C modulates EGFR trafficking between recycling and degradative endosomal compartments, so the antibody is suited to studies of non-canonical receptor regulation rather than activation-loop tyrosine autophosphorylation. Endogenous phospho-receptor signal is captured selectively on whole-cell lysates, with negligible background from unphosphorylated EGFR under standard blotting conditions. Shipped cold on gel packs and intended for storage at -20 °C in working aliquots; repeated freeze-thaw cycles should be avoided to preserve phospho-epitope recognition across the shelf life of the stock.