Anti-EGFR (phospho T678) Antibody

This rabbit polyclonal IgG reagent from Antibodies.com specifically recognizes the epidermal growth factor receptor (EGFR) when phosphorylated on threonine 678, a site in the cytoplasmic tail that is distinct from the classical C-terminal tyrosine autophosphorylation positions such as Y992, Y1068 and Y1173. In published signaling research, threonine 678 has been described as a substrate for p38 MAPK under cellular stress conditions, with modification at this position linked to accelerated receptor internalization and ligand-independent downregulation of surface EGFR.

Supplied as 100 µl of unconjugated antibody validated for western blot on human samples, the reagent gives researchers a tool for monitoring stress- and MAPK-driven EGFR phosphorylation events separately from growth-factor-induced tyrosine phosphorylation. Because recognition depends on the phosphorylated epitope, lysates are typically prepared in the presence of phosphatase inhibitors and freshly resolved by SDS-PAGE before transfer. The unconjugated format allows flexible pairing with HRP-, fluorescent-, or biotin-conjugated secondary antibodies selected to match the chosen detection system. The antibody is reactive with human EGFR and is intended for research use only.